プロトコール(ES その他)

Methods

ES Cells

Establishment of ES cells

(1) TT2 ES Cells from an F1 embryo between C57BL/6 and CBA

A novel ES cell line, TT2, with high germline-differentiating potency.
Yagi T, Tokunaga T, Furuta Y, Nada S, Yoshida M, Tsukada T, Saga Y, Takeda N, Ikawa Y, Aizawa S. (1993)
Anal Biochem. 1993 Oct;214(1):70-6

(2) HK3i ES cells from C57BL/6N embryos

Three Inhibitors of FGF Receptor, ERK and GSK3 Establishes Germline-Competent Embryonic Stem Cells of C57BL/6N Mouse Strain with High Efficiency and Stability.
Kiyonari H, Kaneko M, Abe S and Aizawa S. (2010)
Genesis 2010 May;48(5):317-27.