Reporter mouse
生体モデル開発チームでは、アカデミアに対しレポーターマウスの分与を行なっています。分与に際し、MTAを締結します。分与をご希望の方は、リクエストフォームに記入の上、mutant.bdr(at)riken.jp にお送りください。
リクエストフォームLARGE has recently developed a number of fluorescent reporter mouse lines useful for live imaging of embryos and tissues to aid better understanding of dynamic developmental processes. The following list of reporter mice are available to the scientific community.
Please contact “mutant.bdr(at)riken.jp” to request the reporter mouse line of your interest.
Request FormReporter mouse list
| Applications | Line names | CDB Acc. No. | Ref. |
|---|---|---|---|
| Nucleus | R26R-H2B-EGFP | CDB0203K | 1 |
| Nucleus | R26R-H2B-mCherry | CDB0204K | 1 |
| Nucleus | R26R-H2B-mKeima-Red | CDB0205K | 1 |
| Nucleus | R26-H2B-EGFP | CDB0238K | 1 |
| Nucleus | R26-H2B-mCherry | CDB0239K | 1 |
| Nucleus | R26-H2B-mKeima-Red | CDB0240K | 1 |
| Mitochondria | R26R-Mito-EGFP | CDB0216K | 1 |
| Mitochondria | R26-Mito-EGFP | CDB0251K | 1 |
| Golgiapparatus | R26R-Golgi-EGFP | CDB0211K | 1 |
| Golgiapparatus | R26R-Golgi-mCherry | CDB0212K | 1 |
| Golgiapparatus | R26-Golgi-EGFP | CDB0246K | 1 |
| Golgiapparatus | R26-Golgi-mCherry | CDB0247K | 1 |
| Membrane | R26R-Lyn-Venus | CDB0219K | 1 |
| Membrane | R26R-Display-Venus | CDB0208K | 1 |
| Membrane | R26R-Display-mCherry | CDB0209K | 1 |
| Membrane | R26R-Display-KikGR | CDB0207K | 1 |
| Membrane | R26-Lyn-Venus | CDB0254K | 1 |
| Membrane | R26-Display-Venus | CDB0243K | 1 |
| Membrane | R26-Display-mCherry | CDB0244K | 1 |
| Membrane | R26-Display-KikGR | CDB0242K | 1 |
| Membrane | R26R-Lyn-mCherry | CDB0278K | 2 |
| Membrane | R26-Lyn-mCherry | CDB0287K | 2 |
| Microtubules | R26R-EGFP-Tuba | CDB0210K | 1 |
| Microtubules | R26R-hEMTB-EGFP | CDB0214K | 1 |
| Microtubules | R26R-EB1-EGFP | CDB0213K | 1 |
| Microtubules | R26-EGFP-Tuba | CDB0245K | 1 |
| Microtubules | R26-hEMTB-EGFP | CDB0249K | 1 |
| Microtubules | R26-EB1-EGFP | CDB0248K | 1 |
| Cytoplasm | CAG-KikGR-1 | CDB0201T-1 | 4 |
| Cytoplasm | CAG-KikGR-2 | CDB0201T-2 | 4 |
| Cytoplasm | R26R-ManGeKyou (R26R-MGK) | CDB0222K | 11 |
| Cytoplasm | R26R-EGFP | CDB0297K | 11 |
| Cytoplasm | R26R-nKikGR | CDB0312K | 13 |
| Cytoplasm | R26R-nKikGR | CDB0318K | 13 |
| Actin cytoskeleton | R26R-Venus-Actin | CDB0217K | 1 |
| Actin cytoskeleton | R26R-Venus-Moesin | CDB0221K | 1 |
| Actin cytoskeleton | R26-Venus-Actin | CDB0253K | 1 |
| Actin cytoskeleton | R26-Venus-Moesin | CDB0252K | 1 |
| Actin cytoskeleton | R26R-EGFP-Toripbp4 | CDB0280K | 3 |
| Actin cytoskeleton | R26-EGFP-Toripbp4 | CDB0289K | 3 |
| Tight junction | R26R-ZO1-EGFP | CDB0225K | 12 |
| Tight junction | R26-ZO1-EGFP | CDB0260K | 12 |
| Adherence junction | R26R-PHA7-EGFP | CDB0226K | 9 |
| Adherence junction | R26-PHA7-EGFP | CDB0261K | 9 |
| Nucleus & Membrane | R26R-RG | CDB0227K | 2 |
| Nucleus & Membrane | R26-RG | CDB0237K | 2 |
| Fucci2-G1 | R26R-mCherry-hCdt1(30/120) | CDB0229K | 3 |
| Fucci2-S/G2/M | R26R-mVenus-hGem(1/110) | CDB0230K | 3 |
| Fucci2 | R26p-Fucci2 | CDB0203T | 3 |
| Fucci2-G1 | R26-mCherry-hCdt1(30/120) | CDB0264K | 3 |
| Fucci2-S/G2/M | R26-mVenus-hGem(1/110) | CDB0265K | 3 |
| Wnt signaling pathway | R26-WntVis | CDB0303K | 8 |
| ERK FRET sensor | R26R-EKAREV-NES | CDB0306K | 10 |
| ERK FRET sensor | R26R-EKAREV-NLS | CDB0307K | 10 |
| Basement membranes | R26R-CAG-Nid1-mCherry | CDB0311K | 14 |
| Basement membranes | R26-CAG-Nid1-mCherry mouse line | CDB0320K | 14 |
| Tension FRET sensor | R26R-Strech1 | CDB0054E | 15 |
| Tension FRET sensor | R26R-Strech2 | CDB0055E | 15 |
Publications
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Establishment of conditional reporter mouse lines at ROSA26 locus for live cell imaging.Abe T, Kiyonari H, Shioi G, Inoue K, Nakao K, Aizawa S, Fujimori T.
Genesis.2011 Jul;49(7):579-90.
PMID: 21445964 -
A mouse reporter line to conditionally mark nuclei and cell membranes for in vivo live-imaging.Shioi G, Kiyonari H, Abe T, Nakao K, Fujimori T, Jang CW, Huang CC, Akiyama H, Behringer RR, Aizawa S.
Genesis.2011 Jul;49(7):570-8.
PMID: 21504045 -
Visualization of cell cycle in mouse embryos with Fucci2 reporter directed by Rosa26 promoter.Abe T, Sakaue-Sawano A, Kiyonari H, Shioi G, Inoue K, Horiuchi T, Nakao K, Miyawaki A, Aizawa S, Fujimori T.
Development.2013 Jan 1;140(1):237-46.
PMID: 23175634 -
Blastocyst axis is specified independently of early cell lineage but aligns with the ZP shape.Kurotaki Y1, Hatta K, Nakao K, Nabeshima Y, Fujimori T.
Science.2007 May 4;316(5825):719-23.
PMID: 17446354 -
Reporter mouse lines for fluorescence imaging.Abe T, Fujimori T.
Dev Growth Differ.2013 May;55(4):390-405. Review.
PMID: 23621623 -
Live imaging of early mouse embryos using fluorescently labeled transgenic mice.Abe T, Aizawa S, Fujimori T.
Methods Mol Biol.2013;1052:101-8.
PMID: 23674079 -
Synergistic action of nectins and cadherins generates the mosaic cellular pattern of the olfactory epithelium.Katsunuma S, Honda H, Shinoda T, Ishimoto Y, Miyata T, Kiyonari H, Abe T, Nibu K, Takai Y, Togashi H.
J Cell Biol.2016 Feb 29;212(5):561-75.
PMID: 26929452 -
R26-WntVis reporter mice showing graded response to Wnt signal levels.Takemoto T, Abe T, Kiyonari H, Nakao K, Furuta Y, Suzuki H, Takada S, Fujimori T, Kondoh H.
Genes Cells.2016 Jun;21(6):661-9.
PMID: 27030109 -
Apical constriction in distal visceral endoderm cells initiates global, collective cell rearrangement in embryonic visceral endoderm to form anterior visceral endoderm.Shioi, G., Hoshino, H., Abe, T., Kiyonari, H., Nakao, K., Meng, W., Furuta, Y., Fujimori, T., and Aizawa, S.
Developmental biology2017 Sep 1;429(1):20-30
PMID: 28712875 -
Live-cell FRET imaging reveals a role of extracellular signal-regulated kinase activity dynamics in thymocyte motilityKonishi, Y., Terai, K., Fruta, Y., Kiyonari, H., Abe, T., Ueda, Y., Kinashi, T., Hamazaki, Y., Takaori-Kondo, A., and Matsuda, M.
iSicence2018 Dec 21;10:98-113 -
ROSA26 reporter mouse lines and image analyses reveal the distinct region-specific cell behaviors in the visceral endodermAbe, T., Kutsuna, N., Kiyonari, H., Furuta, Y.,and Fujimori, T.
Development2018 Nov 21;145(22)
PMID: 30327323 -
Dynamic organelle localization and cytoskeletal reorganization during preimplantation mouse embryo development revealed by live imaging of genetically encoded fluorescent fusion proteinsKiyonari, H., Kaneko, M., Abe, T., Shioi, G., Aizawa, S., Furuta, Y., Fujimori, T.
genesis2018 Feb; 57(2):e23277
PMID: 30597711 -
Tracing the origin of hair follicle stem cellsMorita, R., Sanzen, N., Sasaki, H., Hayashi, T., Umeda, M., Yoshimura, M., Yamamoto, T., Shibata, T., Abe, T., Kiyonari, H., Furuta, Y., Nikaido, I., Fujiwara, H.
Nature2021 Jun; 594(7864):547-552
PMID: 34108685 -
Visualization of basement membranes by a nidogen-based fluorescent reporter in miceFutaki S, Horimoto A, Shimono C, Norioka N, Taniguchi Y, Hamaoka H, Kaneko M, Shigeta M, Abe T, Sekiguchi K, Kondo Y.
Matrix Biol Plus2023 Jun; 18:100133
PMID: 37131404 -
In situ FRET measurement of cellular tension using conventional confocal laser microscopy in newly established reporter mice expressing actinin tension sensorWang J, Maeda E, Tsujimura Y, Abe T, Kiyonari H, Kitaguchi T, Yokota H, Matsumoto T.
Sci Rep.2023 13(1):22729.
PMID: 38123655